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After confirming that the concentration of NADPH in our lysates was within the linear detection range of the assay (Fig 3A), we treated LPS-stimulated (6 h) RAW264.7 Ms with 2-DG and diamide and quantified NADPH kinetics by obtaining lysates at 5-minute intervals for a total of 25 minutes (Fig 3B)

The GH-IGF-I axis and the cardiovascular system: clinical implications

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Community resources describe pulling the vial out, drawing the dose, and returning it immediately